DNA

Part:BBa_K3953004:Design

Designed by: Ethan Lorence   Group: iGEM21_Pittsburgh   (2021-10-21)


fimH HIS Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 872
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 872
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 171
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 872
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 872
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Accounting for the fimH leader sequence when placing the His tag, resulting in placement at the 73rd amino acid.


Source

fimH gene from E. coli

References